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Development of HP-TLC method for rapid quantification of sugars, catechins, phenolic acids and saponins to assess Yam (Dioscorea spp.) tuber flour quality

Lebot Vincent, Malapa Roger, Molisalé Tari. 2019. Plant Genetic Resources.
Development of HP-TLC method for rapid quantification of sugars, catechins, phenolic acids and saponins to assess Yam (Dioscorea spp.) tuber flour quality
ARTICLE, (2019 ) - PUBLISHEDVERSION - English (en-GB)
Plant Genetic Resources
OPENACCESS - Cirad license, info:eu-repo/semantics/restrictedAccess, https://agritrop.cirad.fr/mention_legale.html.
Audience : RESEARCHERS
NIAB
Subject
F30 - Génétique et amélioration des plantes, F60 - Physiologie et biochimie végétale, Q04 - Composition des produits alimentaires, Igname, Composition chimique, Sucre, Technique analytique
Domains
Agriculture, Biologie, Sciences du vivant, Sciences exactes, Chimie, Agronomie
Description
Eight yam species (Dioscorea spp.) represented by 522 accessions (landraces and hybrids) were analysed for the individual sugars, catechins, phenolic acids and saponins contents of their tuber flours. Maltose, sucrose, glucose and fructose were quantified. Reducing sugars mean values were highly variable within species and ranged from 0.16%dry weight (DW) (SD ± 0.12) in D. dumetorum to 3.15%DW (±2.49) in D. esculenta. Maltose was detected only in D. esculenta. Chlorogenic acid, gallic acid and other phenolic acids ranged from 4.33 mg/g DW in D. bulbifera to 4.87 mg/g in D. alata and 9.55 mg/g in D. nummularia but were not detected in other species. Catechins (epicatechin and catechin) were highest in D. bulbifera bulbils (25.18 mg/g) and tubers (6.96 mg/g), and lowest in D. esculenta (0.32 mg/g). Their content is significantly correlated with dark flour colour and they most likely contribute to the oxidation of tuber flesh. Saponins (dioscin and gracillin) were quantified in only two species: D. cayenensis (5.94 mg/g, SD ± 3.78) and D. esculenta (3.74 mg/g, SD ± 3.72). Varietal selection may tend to reduce sugars levels and increase secondary metabolites with bioactive properties. HP-TLC is a suitable technique for the rapid quantification of compounds related to yam tuber flour quality.
Keywords
catechin, dioscin, Dioscorea, epicatechin, gracillin, tuber oxidation
Language
English (en-GB)
Creators
Lebot Vincent, Malapa Roger, Molisalé Tari
Contributors
Sources
Plant Genetic Resources: Characterization and Utilization (2019) 17(1); 62–72
Relation
https://doi.org/10.1017/S1479262118000333
10.1017/S1479262118000333
info:eu-repo/semantics/altIdentifier/doi/10.1017/S1479262118000333
info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1017/S1479262118000333
Coverage
Name of newspaper
Plant Genetic Resources