Subject
Pacific, dengue, molecular evolution, phylogeny, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy

Domains
Santé, Biologie, Sciences du vivant

Description

International audience Dengue virus (DENV) serotype-2 was detected in the South Pacific region in 2014 for the first time in 15 years. In 2016-2020, DENV-2 re-emerged in French Polynesia, Vanuatu, Wallis and Futuna, and New Caledonia, co-circulating with and later replacing DENV-1. In this context, epidemiological and molecular evolution data are paramount to decipher the diffusion route of this DENV-2 in the South Pacific region. In the current work, the E gene from 23 DENV-2 serum samples collected in Vanuatu, Fiji, Wallis and Futuna, and New Caledonia was sequenced. Both maximum likelihood and Bayesian phylogenetic analyses were performed. While all DENV-2 strains sequenced belong to the Cosmopolitan genotype, phylogenetic analysis suggests at least three different DENV-2 introductions in the South Pacific between 2017 and 2020. Strains retrieved in these Pacific Islands Countries and Territories (PICTs) in 2017-2020 are phylogenetically related, with strong phylogenetic links between strains retrieved from French PICTs. These phylogenetic data substantiate epidemiological data of the DENV-2 diffusion pattern between these countries.

Keywords

Language
English (en-GB)

Creators
Inizan, Catherine, O'Connor, Olivia, Worwor, George, Cabemaiwai, Talica, Grignon, Jean-Claude, Girault, Dominique, Minier, Marine, Prot, Matthieu, Ballan, Valentine, Pakoa, George Junior, Grangeon, Jean-Paul, Guyant, Philippe, Lepers, Christelle, Faktaufon, Daniel, Sahukhan, Aalisha, Merilles, Onofre Edwin, , Gourinat, Ann-Claire, Simon-Loriere, Etienne, Dupont-Rouzeyrol, Myrielle

Contributors
Dengue et Arbovirose (URE-DA) ; Institut Pasteur de Nouvelle-Calédonie ; Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Ministry of health [Vanuatu], Fiji Centre for Communicable Disease Control [Suva, Fidji], Hôpital de Sia [Wallis], Génomique évolutive des virus à ARN - Evolutionary genomics of RNA viruses ; Institut Pasteur [Paris], Direction des affaires sanitaires et sociales de Nouvelle-Calédonie, WHO Country Liaison Office [Port Vila, Vanuatu] ; Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Pacific community (SPC), Centre hospitalier territorial Gaston-Bourret [Nouméa], This research was funded by an internal seed-funding from the Institut Pasteur of New Caledonia and by Agence Nationale de la Recherche under DENWOLution Grant Agreement no. ANR-19-CE35-0001-01. E.S.-L. acknowledges funding from the INCEPTION program (Investissements d’Avenir grant ANR-16-CONV-0005). Shipment of samples collected in Vanuatu and analysis conducted at Institut Pasteur of New Caledonia were supported by WHO funding for national public health surveillance with support from Department of Foreign Affairs and Trade (DFAT), Australia. Shipment and testing of samples collected in Fiji were funded by SPC with support from DFAT., We acknowledge the Vila Central Hospital (VCH) laboratory technicians Sero Kalkie and Leiwia Dick for their contribution to samples collection, processing and shipment in Vanuatu. We thank Senior Public Health Surveillance Officer Vanua Sikon who contributed to the coordination of samples collection between health facilities and the VCH laboratory. We acknowledge the Pacific Public Health Surveillance Network for the implementation of the pipeline for laboratory specimen shipment and testing. We thank Louis Cognet for his contribution to samples handling and preparation for sequencing. We warmly thank Yvon Cavaloc and the sequencing platform at the University in New Caledonia., ANR-16-CONV-0005,INCEPTION,Institut Convergences pour l'étude de l'Emergence des Pathologies au Travers des Individus et des populatiONs(2016)

Sources
ISSN: 1999-4915, Viruses, https://hal-riip.archives-ouvertes.fr/pasteur-03039686, Viruses, MDPI, 2020, 12 (10), pp.1081. ⟨10.3390/v12101081⟩

Relation
info:eu-repo/semantics/altIdentifier/doi/10.3390/v12101081
info:eu-repo/semantics/altIdentifier/pmid/32992973

Coverage
Vanuatu

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