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Rapid quantitative determination of maltose and total sugars in sweet potato (Ipomoea batatas L. [Lam.]) varieties using HPTLC

Lebot Vincent. 2017. Journal of Food Science and Technology.
Rapid quantitative determination of maltose and total sugars in sweet potato (Ipomoea batatas L. [Lam.]) varieties using HPTLC
ARTICLE, (2017 ) - PUBLISHEDVERSION - English (en-GB)
Journal of Food Science and Technology
RESTRICTEDACCESS - Cirad license.
Audience : RESEARCHERS
Association of Food Scientists & Technologists (India)
Sujet
Q04 - Composition des produits alimentaires, F60 - Physiologie et biochimie végétale, U30 - Méthodes de recherche, F30 - Génétique et amélioration des plantes, Patate douce, Ipomoea batatas, Maltose, Teneur en glucides, Analyse quantitative, Variété, Technique analytique, Composition chimique, Composition des aliments, Chromatographie en couche mince
Domaines
Agriculture
Description
When a raw sweet potato root is analysed, only sucrose, glucose and fructose are present but during cooking, starch is hydrolysed into maltose giving the sweet flavour to cooked roots. This study aimed at developing an HPTLC protocol for the rapid quantitative determination of maltose and total sugars in four commercial varieties and to compare them to 243 hybrids grouped by flesh colour (white, orange, purple). In commercial varieties, mean maltose content varied from 10.26 to 15.60% and total sugars from 17.83 to 27.77% on fresh weight basis. Hybrids showed significant variation in maltose content within each group, with means ranging from 7.65% for white-fleshed, to 8.53% in orange- and 11.98% in purple-fleshed. Total mean sugars content was 20.24, 22.11 and 26.84% respectively for white, orange and purple flesh hybrids. No significant correlations were detected between individual sugars but maltose and total sugars content were highly correlated. Compared to the best commercial variety (Baby), 25 hybrids (10.3%) presented a higher maltose content and 40 (16.5%) showed a higher total sugars content. HPTLC was observed as an attractive, cost efficient, high-throughput technique for quantitating maltose and total sugars in sweet potatoes. Perspectives for improving sweet potato quality for consumers' requirements are also discussed. (Résumé d'auteur)
Mots-clés
Clonal generation, Early screening, Hybrids, Phenotypic recurrent selection, Sweet potato
Langue
English (en-GB)
Auteurs
Lebot, Vincent
Contributeurs
Sources
J Food Sci Technol (March 2017) 54(3):718–726
Couverture
Vanuatu
Nom du journal
Journal of Food Science and Technology