High-Throughput Analysis of Flavokawains in Kava (Piper methysticum Forst. f.) Roots, Chips and Powders and Correlations with Their Acetonic Extracts Absorbance
Lebot Vincent, Kaoh Juliane, Legendre Laurent. 2020-08. .
ARTICLE, (2020-08 ) - PUBLISHEDVERSION - English (en-GB)
OPENACCESS -
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Audience : OTHER
HAL CCSD, Springer
Sujet
Chalcones, Cytoxicity, Flavokawains, Kavalactones, HPTLC, Plant partitioning, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Domaines
Biologie, Kava, Sciences exactes
Description
International audience Kava is a non-alcoholic beverage prepared by cold water extraction of the ground roots and stumps ofPiper methysticumForst. f. Kava contains flavokawains (FKs) which have been suspected of being potentially cytotoxic. Current HPLC protocols are not adapted to high-throughput quantification before export. The objectives of the present study were (i) to analyse with HPTLC the individual FKs in roots, stump, stems and peelings of four varieties grown in a controlled environment; (ii) to quantify FKs in 1053 commercial samples exported from Vanuatu in 2017-18-19 (370 roots, 381 chips and 302 powders) and (iii) to assess the efficiency of a colorimetric test for routine control. HPTLC plate scanning at 355 nm offered good linearity for three FKs withR(2)> 0.99 and RSD < 3.0%. High total FKs (> 14 mg/g DW) were found in poor-quality varieties and in peelings unsuitable for consumption. Plant parts known for their good quality, such as roots and peeled stumps of noble varieties, presented low total FKs (< 7 mg/g). Great variation was observed in exported roots (2.53-24.56 mg/g), chips (2.73-18.03 mg/g) and powders (2.92-16.41 mg/g). HPTLC proved reproducible for the high-throughput quantification of FKs in kava. A positive relationship was confirmed between the absorbance of the acetonic extract and the total FKs (R-2=0.5211) (n =1053). Multivariate analyses revealed that in roots, chips and powders, the three FKs are significantly correlated with high absorbance values. The absorbance of the acetonic extract gives a fair assessment of the FK content in kava products.
Auteurs
Lebot, Vincent, Kaoh, Juliane, Legendre, Laurent
Contributeurs
Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Département Systèmes Biologiques (Cirad-BIOS) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Coconut Division (VARTC) ; Vanuatu Agricultural Research and Technical Center, Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM) ; Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Sources
ISSN: 1936-9751, EISSN: 1936-976X, Food Analytical Methods, https://hal.inrae.fr/hal-03152067, Food Analytical Methods, 2020, 13 (8), pp.1583-1593. ⟨10.1007/s12161-020-01781-9⟩
Relation
info:eu-repo/semantics/altIdentifier/doi/10.1007/s12161-020-01781-9
