In-depth characterization of diazotroph activity across the western tropical South Pacific hotspot of N2 fixation (OUTPACE cruise)
Bonnet Sophie, Caffin Mathieu, Berthelot Hugo, Grosso Olivier, Benavides Mar, Helias Nunige Sandra, Guieu Cécile, Stenegren Mar, Foster Rachel Ann. 2018. .
ARTICLE, (2018 ) - PUBLISHEDVERSION - English (en-GB)
OPENACCESS -
http://creativecommons.org/licenses/by/, info:eu-repo/semantics/OpenAccess.
Audience : OTHER
HAL CCSD, European Geosciences Union
Sujet
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
Domaines
Ecologie, Sciences de l'environnement, Environnement, Sciences exactes
Description
International audience Here we report N 2 fixation rates from a ∼ 4000 km transect in the western and central tropical South Pacific , a particularly undersampled region in the world ocean. Water samples were collected in the euphotic layer along a west to east transect from 160 • E to 160 • W that covered contrasting trophic regimes, from oligotro-phy in the Melanesian archipelago (MA) waters to ultra-oligotrophy in the South Pacific Gyre (GY) waters. N 2 fixation was detected at all 17 sampled stations with an average depth-integrated rate of 631 ± 286 µmol N m −2 d −1 (range 196-1153 µmol N m −2 d −1) in MA waters and of 85 ± 79 µmol N m −2 d −1 (range 18-172 µmol N m −2 d −1) in GY waters. Two cyanobacteria, the larger colonial filamen-tous Trichodesmium and the smaller UCYN-B, dominated the enumerated diazotroph community (> 80 %) and gene expression of the nifH gene (cDNA > 10 5 nifH copies L −1) in MA waters. Single-cell isotopic analyses performed by nanoscale secondary ion mass spectrometry (nanoSIMS) at selected stations revealed that Trichodesmium was always the major contributor to N 2 fixation in MA waters, accounting for 47.1-83.8 % of bulk N 2 fixation. The most plausible environmental factors explaining such exceptionally high rates of N 2 fixation in MA waters are discussed in detail, emphasizing the role of macro-and micro-nutrient (e.g., iron) availability , seawater temperature and currents.
Mots-clés
Environnement, Environment, Océanographie, Ecology
Auteurs
Bonnet, Sophie, Caffin, Mathieu, Berthelot, Hugo, Grosso, Olivier, Benavides, Mar, Helias Nunige, Sandra, Guieu, Cécile, Stenegren, Mar, Foster, Rachel Ann
Contributeurs
Institut méditerranéen d'océanologie (MIO) ; Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) ; Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) ; Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'océanographie de Villefranche (LOV) ; Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV) ; Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Stockholm University, The LEFE-CyBER program (CNRS-INSU), the Institut de Recherche pour le Développement (IRD), the GOPS program (IRD) and the CNES (BC T23, ZBC 4500048836), ANR-14-CE01-0007,OUTPACE,Sequestration océanique du CO2 et fixation d'azote atmosphérique dans l'océan oligotrophe (campagne océanographique OUTPACE).(2014), European Project: 625185,EC:FP7:PEOPLE,FP7-PEOPLE-2013-IEF,DIADOM(2014)
Sources
ISSN: 1726-4170, EISSN: 1726-4189, Biogeosciences, https://hal-amu.archives-ouvertes.fr/hal-02024176, Biogeosciences, 2018, 15 (13), pp.4215-4232. ⟨10.5194/bg-15-4215-2018⟩
Relation
info:eu-repo/semantics/altIdentifier/doi/10.5194/bg-15-4215-2018
info:eu-repo/grantAgreement/EC/FP7/625185/EU/Interactions between nitrogen-fixers (diazotrophs) and dissolved organic matter in the ocean/DIADOM